منابع مشابه
Deciphering NAD-Dependent Deacetylases
each region through immunofluorescence, cross-linking Pacific Hall 0347 and chromatin immunoprecipitation, and biochemical 9500 Gilman Drive purification. SIR2 is not itself essential for viability, but La Jolla, California 92093 sir2⌬ null mutants have a number of phenotypes. In addition to transcriptional silencing defects, sir2⌬ strains have increased rates of recombination within Pundits an...
متن کاملTen years of NAD-dependent SIR2 family deacetylases: implications for metabolic diseases.
Since the discovery of NAD-dependent deacetylase activity of the silent information regulator-2 (SIR2) family ('sirtuins'), many exciting connections between protein deacetylation and energy metabolism have been revealed. The importance of sirtuins in the regulation of many fundamental biological responses to various nutritional and environmental stimuli has been firmly established. Sirtuins ha...
متن کاملStructural basis for nicotinamide cleavage and ADP-ribose transfer by NAD(+)-dependent Sir2 histone/protein deacetylases.
Sir2 enzymes are broadly conserved from bacteria to humans and have been implicated to play roles in gene silencing, DNA repair, genome stability, longevity, metabolism, and cell physiology. These enzymes bind NAD(+) and acetyllysine within protein targets and generate lysine, 2'-O-acetyl-ADP-ribose, and nicotinamide products. To provide structural insights into the chemistry catalyzed by Sir2 ...
متن کاملThe silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases.
Homologs of the chromatin-bound yeast silent information regulator 2 (SIR2) protein are found in organisms from all biological kingdoms. SIR2 itself was originally discovered to influence mating-type control in haploid cells by locus-specific transcriptional silencing. Since then, SIR2 and its homologs have been suggested to play additional roles in suppression of recombination, chromosomal sta...
متن کاملNonisotopic substrate for assaying both human zinc and NAD+-dependent histone deacetylases.
Histone deacetylases (HDACs) are involved in the regulation of transcription and their inhibitors are a promising class of new anticancer drugs. We have previously reported Boc(Ac)Lys-AMC, also termed MAL, as a fluorescent substrate for HDACs. Now we present a modification of MAL called Z-MAL that is characterized by an increased rate of conversion by histone deacetylases of classes I and II an...
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ژورنال
عنوان ژورنال: Cell
سال: 2001
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(01)00305-1